The spider genus Bistriopelma Kaderka, 2015 is endemic to Peru and includes three species
known to date: Bistriopelma lamasi Kaderka, 2015, B. matuskai Kaderka, 2015 and B. titicaca Kaderka,
2017. The present contribution diagnoses, describes and illustrates two new species of Bistriopelma
from Peru: B. peyoi sp. nov. and B. kiwicha sp. nov., both based on male and female. The male of
B. peyoi sp. nov. is provided with a remarkable thoracic horn, and the species is the first horned tarantula for this genus. An updated distribution map for all known species is presented.
Acanthoscurria sacsayhuaman sp. nov. (Araneae: Theraphosidae) is described based on a male from Cusco,
Peru. The new species is characterized by the absence of stridulatory bristles on retrolateral face of palpal
trochanter. Moreover, it can be distinguished by morphology of the male palpal bulb and tibial apophysis of the
first pair of legs. The genus is recorded for the first time for Peru.