Holothele waikoshiemi sp. n. (Theraphosidae, Ischnocolinae) is described based
on two females and a juvenile from Venezuela. The new species is distinguished
by leg I being the longest limb, incrassate tibia I and spermathecae shape.
The genus Guyruita gen. nov. and two new species from Brazil are described. Holothele waikoshiemi (Bertani & Araújo, 2005) from Venezuela is transferred here to the new genus. Guyruita gen. nov. differs from the remaining Ischnocolinae by the following features: labium densely occupied by a lot of cuspules (more than 100), intercheliceral intumescence absent, posterior sternal sigilla remote from margin, tarsal claws without teeth, tarsal scopula I-II undivided (tarsus II with a line of sparse setae, which does not divide the scopula), III-IV divided.